Journal: Cell Reports Medicine

Article Title: mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy

doi: 10.1016/j.xcrm.2023.100978

Figure Lengend Snippet:

Article Snippet: Anti-mouse affinity purified IL-22 , R and D Systems , Polyclonal; Cat# AF582, RRID: AB_355457.

Techniques: In Vivo, Activation Assay, Affinity Purification, Virus, Recombinant, Staining, Membrane, Plasmid Preparation, SYBR Green Assay, Cell Isolation, Enzyme-linked Immunosorbent Assay, RNA sequencing, Transgenic Assay, Control, Negative Control, Retroviral, Software

Journal: Inflammatory bowel diseases

Article Title: Cross-talk between RORγt+ innate lymphoid cells and intestinal macrophages induces mucosal IL-22 production in Crohn's disease.

doi: 10.1097/MIB.0000000000000105

Figure Lengend Snippet: FIGURE 3. A, IL-23 secretion of sorted ILCs populations cocultured with or without macrophages, as determined by enzyme-linked immunosorbent assay (ELISA), n ¼ 3. B, The open bars show IL-22 secretion of sorted cells cocultured with macrophages under LPS stimulation measured by ELISA. The filled bars show sorted cells co- cultured with macrophages and stimulated with LPS and anti-IL-23 antibody (10 mg/mL), n ¼ 3. C, IL-23 receptor messenger RNA was quantified and normalized to 18S RNA expression, n ¼ 12. *P , 0.05.

Article Snippet: The concentration of IL-22 in cell culture supernatants of patients was assayed using a human IL-22 Quantikine enzymelinked immunosorbent assay kit (R&D Systems) with a detection limit of 15.6 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, RNA Expression

Journal: Inflammatory bowel diseases

Article Title: Cross-talk between RORγt+ innate lymphoid cells and intestinal macrophages induces mucosal IL-22 production in Crohn's disease.

doi: 10.1097/MIB.0000000000000105

Figure Lengend Snippet: FIGURE 5. A, The percentage of each population from inflamed lesions (n ¼ 23) and noninflamed lesions (n ¼ 20). B, The open bars show IL-22 secretion levels of ILCs cocultured with macrophages from inflamed lesions. The filled bars show ILCs cocultured from noninflamed lesions in the same individuals. The secretion levels were measured by enzyme-linked immunosorbent assay, n ¼ 4. C, The open bars show IL-23 receptor expression of ILCs from inflamed lesions. The filled bars show IL-23 receptor expression of ILCs from noninflamed lesions. IL-23 receptor mes- senger RNA was quantified and normalized to 18S RNA expression, n ¼ 5. *P , 0.05. NS, not significant.

Article Snippet: The concentration of IL-22 in cell culture supernatants of patients was assayed using a human IL-22 Quantikine enzymelinked immunosorbent assay kit (R&D Systems) with a detection limit of 15.6 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, RNA Expression

Journal: Emerging Microbes & Infections

Article Title: Multidrug-resistant tuberculosis (MDR-TB) strain infection in macaques results in high bacilli burdens in airways, driving broad innate/adaptive immune responses

doi: 10.1038/s41426-018-0213-z

Figure Lengend Snippet: a Representative flow cytometry data (left) show percentages of CD3+ T effector cells producing IFN-γ (upper panel) and perforin in PBMCs from MDR-Mtb- and Erdman-infected macaques at weeks 3 and 6, respectively. Data are represented as the mean ± SEM. b Representative flow cytometry data (left) show percentages of CD3+ T effector cells producing IL-17 (upper panel) or IL-22 in PBMCs at weeks 3 and 6, respectively. Data are represented as the mean ± SEM. c The data represent the mean percentages ± SEM of CD3+ T effector cells producing IFN-γ and perforin, TNF-α, IL-17, and IL-22 in BALF at week 6. d The data show the mean numbers or percentages ± SEM of CD4+ CD25+ Foxp3+ T cells in blood and BAL and those of CD8+ CD25+ Foxp3+ T cells in BAL at weeks 3 and 6 after infection. e Data are represented as the mean numbers or percentages ± SEM of CD8+ IFN-γ+ T cells in blood and BALF measured by ICS with or without PPD stimulation at different time points. Similar data trends for CD4+ IFN-γ+ T cells in blood and BALF measured by ICS with or without PPD stimulation (data not shown). Data were derived from up to six MDR-Mtb-infected and nine Erdman-infected macaques and were analyzed by the Mann–Whitney test (nonparametric method). * p < 0.05, ** p < 0.01

Article Snippet: The following antibodies were used for culturing or surface and intracellular cytokine staining (ICS) for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-PB (SP34-2, BD), CD4-BV510 (L200, BD), CD8-PB (RPA-T8, BD), IFN-γ-APC (4S.B3, BD), IFN-γ Brilliant Violet 711 (4S.B3, Biolegend), TNF-α-PE (MAb11, BD), TNF-α-PE-Cy7 (MAb11, BD), IL-17-PE (eBio64CAP17, eBioscience), IL-22-biotinylated (anti-human IL-22, RD), Streptavidin-Pacific blue (Invitrogen), Perforin-biotinylated (Pf-344, Mabtech), Caspase 3-AF647 (C92-605,BD), and anti-Vγ2-FITC (7A5, Pierce).

Techniques: Flow Cytometry, Infection, Derivative Assay, MANN-WHITNEY

Journal: Cell

Article Title: Commensal Microbiota Promote Lung Cancer Development via γδ T Cells

doi: 10.1016/j.cell.2018.12.040

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cells were serum-starved for 24 hours (1% FBS) before treated with recombinant human amphiregulin (Sigma-Aldrich) or recombinant mouse IL-22 (R&D Systems) at the indicated dose, or left untreated as controls.

Techniques: In Vivo, Purification, Virus, Plasmid Preparation, Recombinant, Enzyme-linked Immunosorbent Assay, Cell Viability Assay, Sequencing