Journal: Cell Reports Medicine

Article Title: mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy

doi: 10.1016/j.xcrm.2023.100978

Figure Lengend Snippet:

Article Snippet: Anti-mouse affinity purified IL-22 , R and D Systems , Polyclonal; Cat# AF582, RRID: AB_355457.

Techniques: In Vivo, Activation Assay, Affinity Purification, Virus, Recombinant, Staining, Membrane, Plasmid Preparation, SYBR Green Assay, Cell Isolation, Enzyme-linked Immunosorbent Assay, RNA sequencing, Transgenic Assay, Control, Negative Control, Retroviral, Software

Journal: Inflammatory bowel diseases

Article Title: Cross-talk between RORγt+ innate lymphoid cells and intestinal macrophages induces mucosal IL-22 production in Crohn's disease.

doi: 10.1097/MIB.0000000000000105

Figure Lengend Snippet: FIGURE 3. A, IL-23 secretion of sorted ILCs populations cocultured with or without macrophages, as determined by enzyme-linked immunosorbent assay (ELISA), n ¼ 3. B, The open bars show IL-22 secretion of sorted cells cocultured with macrophages under LPS stimulation measured by ELISA. The filled bars show sorted cells co- cultured with macrophages and stimulated with LPS and anti-IL-23 antibody (10 mg/mL), n ¼ 3. C, IL-23 receptor messenger RNA was quantified and normalized to 18S RNA expression, n ¼ 12. *P , 0.05.

Article Snippet: The concentration of IL-22 in cell culture supernatants of patients was assayed using a human IL-22 Quantikine enzymelinked immunosorbent assay kit (R&D Systems) with a detection limit of 15.6 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, RNA Expression

Journal: Inflammatory bowel diseases

Article Title: Cross-talk between RORγt+ innate lymphoid cells and intestinal macrophages induces mucosal IL-22 production in Crohn's disease.

doi: 10.1097/MIB.0000000000000105

Figure Lengend Snippet: FIGURE 5. A, The percentage of each population from inflamed lesions (n ¼ 23) and noninflamed lesions (n ¼ 20). B, The open bars show IL-22 secretion levels of ILCs cocultured with macrophages from inflamed lesions. The filled bars show ILCs cocultured from noninflamed lesions in the same individuals. The secretion levels were measured by enzyme-linked immunosorbent assay, n ¼ 4. C, The open bars show IL-23 receptor expression of ILCs from inflamed lesions. The filled bars show IL-23 receptor expression of ILCs from noninflamed lesions. IL-23 receptor mes- senger RNA was quantified and normalized to 18S RNA expression, n ¼ 5. *P , 0.05. NS, not significant.

Article Snippet: The concentration of IL-22 in cell culture supernatants of patients was assayed using a human IL-22 Quantikine enzymelinked immunosorbent assay kit (R&D Systems) with a detection limit of 15.6 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, RNA Expression